Journal: Frontiers in Genetics

Article Title: Genomic epidemiology of Mycobacterium avium subsp. paratuberculosis isolates from Canadian dairy herds provides evidence for multiple infection events

doi: 10.3389/fgene.2023.1043598

Figure Lengend Snippet: Mutations identified in mce proteins in reference to the MAP K-10 Genome.

Article Snippet: The number of MAP bacteria excreted in feces was evaluated by qPCR using standard curves of the MAP K-10 strain (ATCC BAA-968D-5) made with nine serial dilutions (5 and 2 fold, alternatively) from 10 pg to 0.001 pg (2,000–0.2 genomic copies, respectively), based on a genome size of 4.83 Mb.

Techniques: Variant Assay, Mutagenesis

Journal: Frontiers in Genetics

Article Title: Genomic epidemiology of Mycobacterium avium subsp. paratuberculosis isolates from Canadian dairy herds provides evidence for multiple infection events

doi: 10.3389/fgene.2023.1043598

Figure Lengend Snippet: Mutations identified in PE/PPE proteins in reference to the MAP K-10 Genome.

Article Snippet: The number of MAP bacteria excreted in feces was evaluated by qPCR using standard curves of the MAP K-10 strain (ATCC BAA-968D-5) made with nine serial dilutions (5 and 2 fold, alternatively) from 10 pg to 0.001 pg (2,000–0.2 genomic copies, respectively), based on a genome size of 4.83 Mb.

Techniques: Variant Assay, Mutagenesis

Journal: Frontiers in Genetics

Article Title: Genomic epidemiology of Mycobacterium avium subsp. paratuberculosis isolates from Canadian dairy herds provides evidence for multiple infection events

doi: 10.3389/fgene.2023.1043598

Figure Lengend Snippet: Mutations identified in mmpL proteins in reference to the MAP K-10 Genome.

Article Snippet: The number of MAP bacteria excreted in feces was evaluated by qPCR using standard curves of the MAP K-10 strain (ATCC BAA-968D-5) made with nine serial dilutions (5 and 2 fold, alternatively) from 10 pg to 0.001 pg (2,000–0.2 genomic copies, respectively), based on a genome size of 4.83 Mb.

Techniques: Variant Assay, Mutagenesis

Journal: Journal of dairy science

Article Title: Systematic assessment of the reliability of quantitative PCR assays targeting IS900 for the detection of Mycobacterium avium ssp. paratuberculosis presence in animal and environmental samples.

doi: 10.3168/jds.2023-24566

Figure Lengend Snippet: Figure 3. Performance of the 6 qPCR designs using different amounts of MAP K-10 DNA diluted in dilution buffer (A) or fecal DNA extract (B). A serial dilution of DNA from the MAP K-10 reference strain was used to construct a standard curve including 10, 20, 100, 200, 1,000, 2,000, and 10,000 genome equivalents of MAP. The different qPCR assay designs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine,” were analyzed using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means of the qPCR Cycle of quantification (Cq) with different letters differ (P < 0.05).

Article Snippet: Positive control reactions containing 100 genome copies of the MAP K-10 reference strain DNA (ATCC BAA-968) was used to assess plate-to-plate (run-to-run) variability.

Techniques: Serial Dilution, Construct

Journal: Journal of dairy science

Article Title: Systematic assessment of the reliability of quantitative PCR assays targeting IS900 for the detection of Mycobacterium avium ssp. paratuberculosis presence in animal and environmental samples.

doi: 10.3168/jds.2023-24566

Figure Lengend Snippet: Figure 4. Efficiency of qPCR reaction (%Eff) of the 6 qPCR de- signs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine.” Each %Eff was calculated from the slope of a standard curve produced using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means with different letters differ (P < 0.05). The asterisks report a matrix effect (P < 0.05).

Article Snippet: Positive control reactions containing 100 genome copies of the MAP K-10 reference strain DNA (ATCC BAA-968) was used to assess plate-to-plate (run-to-run) variability.

Techniques: Produced

Journal: PLoS ONE

Article Title: Role of TGF-β1 and MAP Kinases in the Antiproliferative Effect of Aspirin in Human Vascular Smooth Muscle Cells

doi: 10.1371/journal.pone.0009800

Figure Lengend Snippet: Panel A: Effect of the ERK MAPK blocker PD98059 when added to ASA (2 mM). DNA synthesis was assessed by BrdU uptake, as stated in . Panel B: Representative blots of ERK MAPK phosphorylation (2 mM). Panel C: Quantification of pooled results. Bar graphs show the mean±S.E.M of n = 4 experiments (cell cultures from 4 different patients). *** P <0.001.

Article Snippet: Inhibitors were: anti-TGF-β1 at 50 μg/ml (R&D Systems, Minneapolis, MN), the p38 MAPK inhibitor SB203580 (10 μM), the ERK MAPK inhibitor PD98059 (10 μM), the ALK-5 blocker at 10 μM SB-431542 (Tocris, Bristol, UK), and anti-CD105 at 1 μg/ml (BD Bioscience, Franklin Lakes, USA), as described .

Techniques: DNA Synthesis, Phospho-proteomics